5.1 Sample protocols for discharges of treated
ballast water through a distinct discharge point fall into two categories,
the first based on specified and replicated volumes and the second
based on flow integration over a specified time. The first entails
taking a specific number of set volumes of the ballast water discharge,
whilst the second takes a continuous sample over a set time period.
The flow integration sampling protocol can be achieved by either continuously
sub-sampling a small amount throughout the entire duration of the
discharge, therefore collecting one sample over time, or taking multiple
sub-samples over a specific time scale (i.e. 5 minutes, 10 minutes
or 15 minutes) repeatedly throughout the discharge, providing a result
for each sub-sample.
5.2 However, for sampling protocols based on specified
and replicated volumes, defining both the number of samples and their
volume to ensure representativeness, takes time. As a representative
sampling procedure is needed to ensure compliance with the BWM Convention, then the flow integration protocols
based on set times should be implemented.
5.3 Using a sampling protocol that continuously
sub-samples small amounts throughout the entire duration of the discharge,
may significantly underestimate the amount of larger organisms (i.e.
organisms greater than or equal to 50 micrometres in minimum dimension)
in the sample due to damage to the organisms held in the cod-end of
the filter. If such a system is used then a protocol for replacing
the cod end needs to be developed.
5.4 The arrangements for detailed analysis should
take into account the requirements of the methods and/or approaches
they intend to use for detailed and/or indicative analysis. Special
consideration should be given and contingencies arranged for sampling
in remote ports, where it is likely to take time to mobilize samplers
and sampling resources.